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Image Search Results
Journal: eLife
Article Title: Functional identification of soluble uric acid as an endogenous inhibitor of CD38
doi: 10.7554/eLife.96962
Figure Lengend Snippet:
Article Snippet: Commercial assay or kit ,
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Colorimetric Assay, Software, Activity Assay
Journal: bioRxiv
Article Title: NAD modulates DNA methylation and cell differentiation
doi: 10.1101/2020.07.31.231555
Figure Lengend Snippet: ( A ) Wright Giemsa staining showing morphological changes between NAD-treated and control cells after four days. ( B ) Increase in the surface markers CD15, CD14 and CD11b upon NAD treatment ( C ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. ( D ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of two biological replicates (n=2). Error bars indicate ± S.D. ( E ) Model showing the molecular mechanism of CEBPA gene reactivation by NAD. CEBPA is epigenetically silenced in K562. DNMT1 ensure a constant methylated status of CEBPA promoter (upper part). The NAD supplementation to K562 cell culture, boosts PARP1 to produce ADP-ribose polymers leading to DNMT1 inhibition (bottom part). The ultimate effect is CEPBA re-activated transcription.
Article Snippet: The cells were then stained with the
Techniques: Staining, Methylation, Cell Culture, Inhibition